Osipov Analysis
Osipov analysis is an innovative method for diagnosing human microbiota, primarily of the small intestine, based on gas chromatography-mass spectrometry (GC-MS).
The method identifies unique microbial markers — biochemical substances formed by microorganisms and significantly different from human cells.
Main features of the method:
- Allows simultaneous detection of more than 50 types of microorganisms: bacteria, viruses, and fungi.
- Sample collection is minimally invasive, using a dry drop blood method, which is also convenient for transport.
- The analysis is performed directly on the biological material without using culture media or biochemical reagents.
- Provides high sensitivity, detecting markers at concentrations as low as 0.01 ng/ml.
- The analysis time does not exceed 3 hours.
- The method is selective, allowing microorganism identification down to species level.
Diagnostic significance:
- Assesses the microbiota of the small intestine, not fully covered by traditional stool analysis methods.
- Applicable for dysbiosis, chronic intestinal inflammations, irritable bowel syndrome, constipation, diarrhea, and after prolonged antibiotic therapy.
- Enables monitoring of microbiota changes over time in patients.
Advantages:
- Universal with respect to different microbial groups.
- Does not require complex laboratory equipment or additional reagents.
- Fast and precise analysis of chemical microbial markers.
Limitations:
- Results are based not on direct microorganism detection but on chemical markers, which may vary in nature, reducing the quantitative accuracy.
- The method requires careful interpretation and is considered an auxiliary diagnostic tool rather than a sole clinical test.
Summary:
Osipov analysis is a powerful modern tool for in-depth study of the small intestine microbiota. It offers high sensitivity and speed but requires comprehensive clinical evaluation of the results.