Osipov Analysis
Osipov analysis is an innovative method for diagnosing human microbiota, primarily of the small intestine, based on gas chromatography-mass spectrometry (GC-MS).
The essence of the method is detecting unique microbial markers — biochemical substances produced by microorganisms that significantly differ from human cells.
Main features of the method:
- Allows simultaneous identification of more than 50 microorganism species: bacteria, viruses, and fungi.
- Sample is collected from a drop of blood using the "dry drop" method, which is minimally invasive and convenient for transportation.
- Analysis is performed directly on the biomaterial without the use of culture media or biochemical reagents.
- Offers high sensitivity — detects markers at concentrations as low as 0.01 ng/mL.
- Analysis time does not exceed 3 hours.
- Selective method enabling microorganism identification down to the species level.
Diagnostic significance:
- Assesses the small intestine microbiota, which traditional stool analysis methods do not fully cover.
- Applicable for dysbiosis, chronic intestinal inflammations, irritable bowel syndrome, constipation, diarrhea, and post-prolonged antibiotic therapy.
- Allows monitoring of microbiota changes in patients over time.
Advantages:
- Universal for different microorganism groups.
- Does not require complex laboratory equipment or additional reagents.
- Fast and accurate analysis of chemical markers of microorganisms.
Limitations:
- Results are based not on direct microorganism detection but on chemical markers, which can have diverse origins, reducing quantitative accuracy.
- The method requires cautious interpretation and is considered an additional diagnostic tool, not a sole clinical test.
Summary:
Osipov analysis is a powerful modern tool for in-depth study of small intestine microbiota. It offers high sensitivity and speed but requires comprehensive result evaluation in clinical practice.